Fluorescent dot counting in interphase cell nuclei
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چکیده
منابع مشابه
Fluorescent dot counting in interphase cell nuclei
Fluorescence in situ hybridization allows the enumeration of chromosomal abnormalities in interphase cell nuclei. This process is called dot counting. To estimate the distribution of chromosomes per cell, a large number of cells have to be analysed, particularly when the frequency of aberrant cells is low. Automation of dot counting is desirable because manual counting is tedious, fatiguing, an...
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Fluorescence in situ hybridization allows the enumeration of chromosomal abnormalities in interphase cell nuclei. This process is called dot counting. To estimate the distribution of chromosomes per cell, a large number of cells have to be analyzed, especially when the frequency of aberrant cells is low. Automation of dot counting is required because manual counting is tedious, fatiguing, and t...
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We have developed a completely automated fluorescence microscope system that can examine 500 cells in approximately 20 minutes to determine the number of labeled chromosomes (seen as dots) in each cell nucleus. This system works with two fluorescent dyes – one for the DNA hybridization dots (e.g. FITC) and one for the cell nucleus (e.g. DAPI). After the stage has moved to a new field the image ...
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ژورنال
عنوان ژورنال: Bioimaging
سال: 1996
ISSN: 0966-9051,1361-6374
DOI: 10.1002/1361-6374(199606)4:2<93::aid-bio7>3.3.co;2-z